Faqs for simple western application and instrumentation. Compass software reports molecular weight, area, percent area and signal to noise for each protein detected. The digital image was analyzed with compass software proteinsimple, and the quantified data of the detected protein were reported as molecular weight and signalpeak intensity. Use of capillary western immunoassay wes for quantification ncbi. And its got all the 21 cfr part 11 compliance tools you need with the new compass authorization server v2. Protein simple has done extensive compatibility testing with multiple buffers and reagents. Make up 1% sds solution % solution dry mass in grams volume in ml x 100 include 1x complete. In most figures, electropherograms are represented as pseudoblots, generated using compass software. Simple western compass software provides both virtual blots and. Compass for ice software automatically processes samples and gives you the final analysis and reporting on your biologics. The western blot has been around in essentially the same form since the late 1970s. That said, the continuous manual intervention to run a western blot is labor intensive and introduces many. Compass for simple western user guide protein simple. The system greatly reduces the variability caused by manual processes in conventional western.
One software package for everything cief cesds compass for ice. Use the email address on your account instead of your user id to sign in with your current password. Fluorchem hd2 upgrade for alphaimager hp for chemiluminescence applications. Absolute quantitation of endogenous proteins with precision and accuracy using a capillary western system. Proteinsimple s simple western technology is covered by issued and pending patents in the u. Jul 01, 20 santa clara, ca, july 1, 20 proteinsimple today released version 2.
Specialized in phosphoprotein signaling, protein purity, protein aggregation, protein fractionation, western blot imaging, gel documentation. Compass software can also be installed on a separa te workstation, such as yo ur desktop computer. Download software and instrument user guides and release notes. Just in case you need it, a cd containing compass for simple western also comes in the box. Cell biosciences proteinsimple cb nanofluidics immunoassay system. Stepshot has recently introduced its newest and most advanced product to quickly create manuals and video tutorials. Here we demonstrate the application of the proteinsimple capillary immunoassay. Choose from a list that includes plate layout, sample peaks, images and much. No messy gels, no transfer tanks, no blots, and no manual analysis.
Looking to purchase one of our products or need support for a product you currently have. Just like it did before, it analyzes simple western size or charge assay data and processes the results for you. And its got all the 21 cfr part 11 compliance tools you need with the new compass. A postmortem on western blots the true cost of western blots. And its got tools for 21 cfr part 11 compliance for traceability. Proteinsimple, the proteinsimple logo, the alpha innotech logo, protein forest, the protein.
An allinone system for western blot and gel imaging duration. Jul 01, 20 the simple western is a western with none of the hassle. The truncated and fulllength p53 peak intensities area under the curve were normalized to that of the vinculin peak, used as a loading control. Has anyone used the proteinsimple wes capillary western blot. Proteinsimple capillary electrophoresis immunoassay was performed according to the proteinsimple manufactures user manual. Late from a large teaching hospital is this lovely. The data was analyzed with inbuilt compass software proteinsimple. Simple westerns separate proteins by size, and precisely manages immunoprobing, incubations, washes and even detection steps by removing the manual, errorprone steps in standard western blots. Data analysis for your cief applications on compass for ice is fast with its easytouse software. Has anyone used the proteinsimple wes capillary western.
Absolute quantitation of endogenous proteins with precision and accuracy using a capillary western system article in analytical biochemistry 4421 july 20 with 5 reads how we measure reads. The compass software used to run wes also allows you to run standard curves and. The nanopro system is cell biosciences nextgeneration immunoassay development platform which builds upon the fundamental technology of its. Proteinsimple 3001 orchard parkway san jose, california, 954 usa. Maurice protein detection instrument user manual maurice.
Compass acts as a router for dicom imagesmessages and hl7 version 2. Complete immunoassay platform includes multiple assay kits, compass analysis software and an expanding library of validated targets save time eliminate timeconsuming manual processes. Simply load up to 96 samples, push a button, walk away and come back to fully analyzed data. We offer complete solutions ranging from design and.
Western blotting is commonly touted as a straightforward, inexpensive technique for protein analysis, and on the surface, it. Proteinsimple s latest compass software enables researchers using the simple western in biopharmaceutical manufacturing and quality. Chr and the manual integration toolbar, zoom in on the baseline of the solvent peak and the smaller unrecognized peak immediately to its right. We offer complete solutions ranging from design and planning over threedimensional visualization to nearly fully automatic cnc manufacturing. All content is posted anonymously by employees working at proteinsimple. Development and characterization of a biomimetic coating. The proteinsimple nanopro system is providing exciting new insights into. Easy transfer of your traditional western blot to wes. Novel small molecules potentiate premature termination.
Compass software is now compass for simple western. Somebody have experience with the wes proteinsimple system. With this software release, not only has proteinsimple fully redesigned the ageold traditional western and replaced it with a fully automated, fully. Rethinking protein tools and helping researchers resolve their protein analysis problems to reveal new insight into proteins and their role in disease. Download the latest version of compass for sw and instrument software for use with simple western systems. Santa clara, ca, july 1, 20 proteinsimple today released version 2. Protocols were optimised for antibody and protein loading. With this software release, not only has proteinsimple. Proteinsimple is part of the protein platforms division of biotechne nasdaq. Has anyone used the proteinsimple wes capillary western blot system. Simple western assays on peggy page 3 peggy user guide. Western blotting is commonly touted as a straightforward, inexpensive technique for protein analysis, and on the surface, it can appear to fit the needs of many labs. Now, with the latest compass software, researchers can easily validate their simple western work to ensure absolute data integrity.
One software package for everything cief cesds compass. Simple western analysis was performed according to the user manual. A proteomic analysis of chondrogenic, osteogenic and. No scope for growth especially in consumables people you work with may or may not be super competent, unfortunately for me it was the latter not compensated according the market. The intensities of each protein were analysed by quantification with compass software proteinsimple. Jess, wes and the proteinsimple logo are trademarks andor registered trademarks of proteinsimple. From the beginning, we have been one of the leading providers of stair manufacturingsoftware worldwide.
Protein detection instrument users manual part 1 details for fcc id 2ahggmaurice made by proteinsimple. Were rethinking protein tools and helping thousands of researchers around the world resolve their protein analysis problems so they can reveal new insight into proteins and their role in disease. The produced chemiluminescence is detected at multiple exposure times and automatically quantified by the compass software. The data shown is for one exposure time and the same data for each exposure time is stored. Proteinsimple simon automatic western blot system with software and accessories. Make up 1% sds solution % solution dry mass in grams volume in ml x 100 include 1x complete mini a 10x stock solution 1 tablet in 1 ml 1% sds is stable for 2 weeks at 28. Compass software is used to run assays and analyze resulting data. Compass software can also be installed on a separa te. Come back to fully analyzed results in as little as 3 hours, not 3 days. Compass software for simple western automate, quantitate, validate. To make a baseline ignore a peak use the none integration tool. Metformin inhibits stromal aromatase expression and tumor. Data analysis for your cief applications on compass for ice is fast with its easytouse software interface.
In brief, whole cell extract samples 800 nglane were mixed with kit provided master mix. Injections displays a list of the sample proteins compass for ice names automatically using the userdefined peak name analysis parameters. Compass for sw and instrument software downloads download the latest version of compass for sw and instrument software for use with simple western systems. The document assumes a basic knowledge of xml and provides instructions on how to customize the proteinpilot software. Proteinsimple simon automatic western blot system with. The nanopro system enables rapid, quantitative analysis of specific proteins in as few as 25 cells per assay.
Here we demonstrate the application of the proteinsimple capillary immunoassay wes method, a gel and blotfree method requiring less sample, antibody and time to run than conventional western blot assay. Proteinsimple platforms simple western which protein is present and how. All of the data stored can be exported as text files or to spreadsheet applications. The simple western is now cgmp ready news provided by. The data shown is for one exposure time and the same data for each exposure time is stored for each run performed. The digital image was analyzed with compass software proteinsimple. No scope for growth especially in consumables people you work with may or may not be super competent, unfortunately for me it was the latter not compensated according the market rate very relaxed on specifications, each issue is taken case to case as opposed to a specific predefined set of specifications people have set up their own cliques, for a newbie its hard to befriend people.
Sample data is displayed by lane in a virtualblot like image similar to traditional results and an electropherogram view example below. Chemiluminescent signal was captured by a chargecoupled device camera and the resulting image was analysed by compass software proteinsimple. When you start the software, the logo is displayed first, and then the log in screen is displayed. Specialized in phosphoprotein signaling, protein purity. It was represented to me as being in good working condition, and if the physical condition is any indication, i believe them. Data were analyzed with proteinsimple compass software. Detailed information is generated on the posttranslational modification status. Compass for simple western software user guide for wes, peggy sue and sally sue. A subset of the sample data obtained by the wes software compass for the run is shown above. Advanced configuration of proteinpilot software ruoidv061665c 5 introduction this document explains the concept of parameter translation used by the paragon algorithm in the proteinpilot software. Compass user manual 2019 laurel bridge software, inc. Actin cytoskeleton assembly regulates collagen production. A proteomic analysis of chondrogenic, osteogenic and tenogenic constructs from ageing mesenchymal stem cells. Were rethinking protein tools and helping thousands of researchers around the world resolve.
An automated immunoblot method for detection of igg. Absolute quantitation of endogenous proteins with precision. Pdf files of the jess, wes and sally suepeggy sue user guides. Please contact your local tobii dynavox reseller for more information based on your location, please select your country from the globe icon at the top of this page. Along with luminolperoxide mix and wash buffer, solutions were placed in their respective locations according to the wes tm plating system, run and analyzed with proteinsimple s compass software. Biotechne today announced the launch of the cesds plus system for its proteinsimple branded maurice platform.
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